Medical Cellular Biology Support
PBMC preparation
We are NATA accredited for PBMC separations and provide support services to clinical trials and researchers requiring processing of blood samples and conducting assays on blood sample fractions. Please enquire and tell us about your requirements and how we can help with your study.
Immunoassays
We are able to provide support for conducting immunoassays of various types please contact us to see if our laboratory can help you with your project requirements.
RNA Seq
Bulk RNAseq
We are able to perform bulk RNA seq on samples from various sources please inquire.
Single Cell RNASeq
Our proprietary single cell RNA seq workflows allow accurate single cell RNA profiling of single cells etc
ERAP Genotyping assay
The Endoplasmic Reticulum Aminopeptidase (ERAP) 1 and ERAP2 genes are responsible for the N terminal trimming of peptides before they are loaded onto HLA molecules for presentation to the immune system. The ERAP1 and ERAP2 genes are extremely polymorphic and key SNPs can be utilised to identify the ERAP1/2 allotypes carried by an individual.
Our lab uses sequence-based typing using the Miseq Illumina platform to target 17 SNPs for ERAP1 and 2 SNPs for ERAP2. For NGS sequencing, multiple indexed amplicons are pooled together prior to sequencing using the Biomek Liquid handlers, which allows sample tracking and maintains data integrity. Sequencing of the amplicons is followed by identification of the specific SNP variants using the In-house developed visualising and analysis tool VGAS.
KIR genotyping
Presence absence (qPCR)
We are able to provide presence absence typing for KIR genotyping
NGS genotyping
We are able to perform more detailed in depth typing of KIR using NGS sequencing.
We offer a sequence typing service for Killer-cell Immunoglobulin-like Receptors (KIR) genes (loci). We have the capacity to type large cohorts of samples to high resolution for 14 gene loci (KIR2DL1, KIR2DL2/L3, KIR2DL4, KIR2DL5A/5B, KIR2DS1, KIR2DS2, KIR2DS3, KIR2DS4, KIR2DS5, KIR3DL1/S1, KIR3DL2, KIR3DL3 and two pseudogenes, KIR2DP1 and KIR3DP1) encoded within a 100-200 Kb region of the Leukocyte Receptor Complex (LRC). Our current cost for KIR allele typing in a collaborative setting for small numbers of samples (<=50) is now $130 USD per sample that covers all the KIR genes mentioned. For further details and to get your project assessed for collaborative gain, and discounts please inquire. Discounts may be available for larger sample sets.
A list of the KIR iGroups can be used to disambiguate the calls if required.
The alleles in any of the KIR genes that have identical nucleotide sequences across the exons encode the peptide binding domains (2 or 3 domains) are grouped together and designated with a lower case “i” (example; 00301, 00302 or 00305 = 00301i, because they are identical at the binding domains).
