Nucleic acid- based assays

Pre- and post-PCR laboratories contain seven Biomek robotic liquid handling systems for automated, high-throughput assays. Additional automation is provided by a formulatrix Mantis and MagMax (pre-PCR). Robotics are used wherever possible in order to minimize manual handling of samples. IIID specializes in HLA and KIR genotyping, TCR and viral genome sequencing and has the capability to perform single cell nucleic acid sequencing. The Pre-PCR laboratory has 3 Biomek robots that are dedicated for DNA and RNA extraction from samples, PCR mastermix preparation and reaction set-up, including digital droplet PCR. The Post-PCR laboratory is used for all activities associated with amplified material and plasmids, using 4 Biomek liquid handling robotics. Sequencing is performed using the Illumina MiSeq sequencing platform.

DNA Sequencing

Nanopore

We offer a service for sequencing on our Oxford Nanopore Technology systems. These platforms have some amazing features as well as their ability to generate very long reads to aid with haplotyping and assemblies.

Parallel sequencing using Illumina sequencing

We offer a full service for sequencing on the Illumina NovaSeq 6000, NextSeq and MiSeq platform (reads up to 2 x 300 bp). We are offering whole genome sequencing, Exome sequencing, RNAseq, CITE-Seq as well as a myriad of other specialised bulk and single cell technologies such as TCR sequencing and targeted gene sequencing including ASHI accredited HLA typing

Please enquire for costs of these services.

Note: The largest component cost in a project may be the bioinformatic handling required to manage sequencing output data.

Other Assays

Please use the navigation above to investigate our other available assays.